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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 17 — Aug. 25, 2014
  • pp: 21079–21087

Directly measuring absolute flow speed by frequency-domain laser speckle imaging

Hao Li, Qi Liu, Hongyang Lu, Yao Li, Hao F. Zhang, and Shanbao Tong  »View Author Affiliations

Optics Express, Vol. 22, Issue 17, pp. 21079-21087 (2014)

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Laser speckle contrast imaging (LSCI) is a simple yet powerful tool to image blood flow. However, traditional LSCI has limited quantitative analysis capabilities due to various factors affecting flow speed evaluation, including illumination intensity, scattering from static tissues, and mathematical complexity of blood flow estimation. Here, we present a frequency-domain laser speckle imaging (FDLSI) method that can directly measure absolute flow speed. In phantom experiments, the measured flow speed agreed well with the preset actual values (10% deviation). Furthermore, in vivo experiments demonstrated that FDLSI was minimally affected by illumination condition changes.

© 2014 Optical Society of America

OCIS Codes
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging
(120.7250) Instrumentation, measurement, and metrology : Velocimetry
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: June 9, 2014
Revised Manuscript: July 26, 2014
Manuscript Accepted: August 2, 2014
Published: August 22, 2014

Virtual Issues
Vol. 9, Iss. 10 Virtual Journal for Biomedical Optics

Hao Li, Qi Liu, Hongyang Lu, Yao Li, Hao F. Zhang, and Shanbao Tong, "Directly measuring absolute flow speed by frequency-domain laser speckle imaging," Opt. Express 22, 21079-21087 (2014)

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