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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 2 — Jan. 27, 2014
  • pp: 1243–1256

Micromirror structured illumination microscope for high-speed in vivo drosophila brain imaging

A. Masson, M. Pedrazzani, S. Benrezzak, P. Tchenio, T. Preat, and D. Nutarelli  »View Author Affiliations


Optics Express, Vol. 22, Issue 2, pp. 1243-1256 (2014)
http://dx.doi.org/10.1364/OE.22.001243


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Abstract

Genetic tools and especially genetically encoded fluorescent reporters have given a special place to optical microscopy in drosophila neurobiology research. In order to monitor neural networks activity, high speed and sensitive techniques, with high spatial resolution are required. Structured illumination microscopies are wide-field approaches with optical sectioning ability. Despite the large progress made with the introduction of the HiLo principle, they did not meet the criteria of speed and/or spatial resolution for drosophila brain imaging. We report on a new implementation that took advantage of micromirror matrix technology to structure the illumination. Thus, we showed that the developed instrument exhibits a spatial resolution close to that of confocal microscopy but it can record physiological responses with a speed improved by more than an order a magnitude.

© 2014 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Microscopy

History
Original Manuscript: September 30, 2013
Revised Manuscript: December 9, 2013
Manuscript Accepted: December 17, 2013
Published: January 13, 2014

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Citation
A. Masson, M. Pedrazzani, S. Benrezzak, P. Tchenio, T. Preat, and D. Nutarelli, "Micromirror structured illumination microscope for high-speed in vivo drosophila brain imaging," Opt. Express 22, 1243-1256 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-2-1243


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