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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 2 — Jan. 27, 2014
  • pp: 1500–1511

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Zhenyuan Yang, Jianhua Chen, Junjie Yao, Riqiang Lin, Jing Meng, Chengbo Liu, Jinhua Yang, Xiang Li, Lihong Wang, and Liang Song  »View Author Affiliations


Optics Express, Vol. 22, Issue 2, pp. 1500-1511 (2014)
http://dx.doi.org/10.1364/OE.22.001500


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Abstract

Many diseases involve either the formation of new blood vessels (e.g., tumor angiogenesis) or the damage of existing ones (e.g., diabetic retinopathy) at the microcirculation level. Optical-resolution photoacoustic microscopy (OR-PAM), capable of imaging microvessels in 3D in vivo down to individual capillaries using endogenous contrast, has the potential to reveal microvascular information critical to the diagnosis and staging of microcirculation-related diseases. In this study, we have developed a dedicated microvascular quantification (MQ) algorithm for OR-PAM to automatically quantify multiple microvascular morphological parameters in parallel, including the vessel diameter distribution, the microvessel density, the vascular tortuosity, and the fractal dimension. The algorithm has been tested on in vivo OR-PAM images of a healthy mouse, demonstrating high accuracy for microvascular segmentation and quantification. The developed MQ algorithm for OR-PAM may greatly facilitate quantitative imaging of tumor angiogenesis and many other microcirculation related diseases in vivo.

© 2014 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 28, 2013
Revised Manuscript: December 1, 2013
Manuscript Accepted: December 5, 2013
Published: January 15, 2014

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Zhenyuan Yang, Jianhua Chen, Junjie Yao, Riqiang Lin, Jing Meng, Chengbo Liu, Jinhua Yang, Xiang Li, Lihong Wang, and Liang Song, "Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo," Opt. Express 22, 1500-1511 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-2-1500


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