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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 3 — Feb. 10, 2014
  • pp: 2807–2820

Conformational analysis of proteins with a dual polarisation silicon microring

J-W. Hoste, S. Werquin, T. Claes, and P. Bienstman  »View Author Affiliations

Optics Express, Vol. 22, Issue 3, pp. 2807-2820 (2014)

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Optical microresonator biosensors have proven to be a valid tool to perform affinity analysis of a biological binding event. However, when these microresonators are excited with a single optical mode they can not distinguish between a thin dense layer of biomolecules or a thick sparse layer. This means the sensor is ”blind” to changes in shape of bound biomolecules. We succeeded in exciting a Silicon-on-Insulator (SOI) microring with TE and TM polarisations simultaneously by using an asymmetrical directional coupler and as such were able to seperately determine the thickness and the density (or refractive index) of a bound biolayer. A proof-of-concept is given by determining both parameters of deposited dielectric layers and by analysing the conformational changes of Bovine Serum Albumin (BSA) proteins due to a change in pH of the buffer.

© 2014 Optical Society of America

OCIS Codes
(130.3120) Integrated optics : Integrated optics devices
(140.4780) Lasers and laser optics : Optical resonators
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:

Original Manuscript: December 16, 2013
Revised Manuscript: January 13, 2014
Manuscript Accepted: January 13, 2014
Published: January 30, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

J-W. Hoste, S. Werquin, T. Claes, and P. Bienstman, "Conformational analysis of proteins with a dual polarisation silicon microring," Opt. Express 22, 2807-2820 (2014)

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