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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 6 — Mar. 24, 2014
  • pp: 6661–6673

High throughput holographic imaging-in-flow for the analysis of a wide plankton size range

Catherine Yourassowsky and Frank Dubois  »View Author Affiliations

Optics Express, Vol. 22, Issue 6, pp. 6661-6673 (2014)

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Abstract: We developed a Digital Holographic Microscope (DHM) working with a partial coherent source specifically adapted to perform high throughput recording of holograms of plankton organisms in-flow, in a size range of 3µm-300µm, which is of importance for this kind of applications. This wide size range is achieved with the same flow cell and with the same microscope magnification. The DHM configuration combines a high magnification with a large field of view and provides high-resolution intensity and quantitative phase images refocusing on high sample flow rate. Specific algorithms were developed to detect and extract automatically the particles and organisms present in the samples in order to build holograms of each one that are used for holographic refocusing and quantitative phase contrast imaging. Experimental results are shown and discussed.

© 2014 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography
(110.3175) Imaging systems : Interferometric imaging

ToC Category:

Original Manuscript: December 11, 2013
Revised Manuscript: February 21, 2014
Manuscript Accepted: February 25, 2014
Published: March 14, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Catherine Yourassowsky and Frank Dubois, "High throughput holographic imaging-in-flow for the analysis of a wide plankton size range," Opt. Express 22, 6661-6673 (2014)

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