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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 7 — Apr. 7, 2014
  • pp: 8150–8155

Microfiber coupler based label-free immunosensor

Lin Bo, Christy Charlton O’Mahony, Yuliya Semenova, Niamh Gilmartin, Pengfei Wang, and Gerald Farrell  »View Author Affiliations


Optics Express, Vol. 22, Issue 7, pp. 8150-8155 (2014)
http://dx.doi.org/10.1364/OE.22.008150


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Abstract

Optical microfibers and related structures which incorporate large evanescent field and minimal size offer new opportunities for biosensing applications. In this paper we report the development of an immunosensor based on a tapered microfiber coupler embedded in a low refractive index polymer. Biomolecules adsorbed on the microfiber coupler surface modify the surrounding refractive index. By immobilizing antigens on the surface of the sensing area, the microfiber coupler was able to operate as a label-free immunosensor to detect specific antibodies. We experimentally demonstrated for the first time the sensing ability of this sensor using a fibrinogen antigen-antibody pair. By monitoring the spectral shift in the wavelength domain, the sensor was shown to be capable of detecting the specific binding between fibrinogen and anti-fibrinogen. The detected signal was found to be proportional to the anti-fibrinogen present.

© 2014 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Sensors

History
Original Manuscript: October 31, 2013
Revised Manuscript: November 1, 2013
Manuscript Accepted: November 27, 2013
Published: April 1, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Lin Bo, Christy Charlton O’Mahony, Yuliya Semenova, Niamh Gilmartin, Pengfei Wang, and Gerald Farrell, "Microfiber coupler based label-free immunosensor," Opt. Express 22, 8150-8155 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-7-8150


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