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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 7 — Apr. 7, 2014
  • pp: 8654–8671

Enhanced 3D spatial resolution in quantitative phase microscopy using spatially incoherent illumination

Pierre Bon, Sherazade Aknoun, Serge Monneret, and Benoit Wattellier  »View Author Affiliations


Optics Express, Vol. 22, Issue 7, pp. 8654-8671 (2014)
http://dx.doi.org/10.1364/OE.22.008654


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Abstract

We describe the use of spatially incoherent illumination to make quantitative phase imaging of a semi-transparent sample, even out of the paraxial approximation. The image volume electromagnetic field is collected by scanning the image planes with a quadriwave lateral shearing interferometer, while the sample is spatially incoherently illuminated. In comparison to coherent quantitative phase measurements, incoherent illumination enriches the 3D collected spatial frequencies leading to 3D resolution increase (up to a factor 2). The image contrast loss introduced by the incoherent illumination is simulated and used to compensate the measurements. This restores the quantitative value of phase and intensity. Experimental contrast loss compensation and 3D resolution increase is presented using polystyrene and TiO2 micro-beads. Our approach will be useful to make diffraction tomography reconstruction with a simplified setup.

© 2014 Optical Society of America

OCIS Codes
(050.1960) Diffraction and gratings : Diffraction theory
(110.0180) Imaging systems : Microscopy
(110.1650) Imaging systems : Coherence imaging
(110.6880) Imaging systems : Three-dimensional image acquisition
(120.5050) Instrumentation, measurement, and metrology : Phase measurement

ToC Category:
Microscopy

History
Original Manuscript: February 10, 2014
Revised Manuscript: March 25, 2014
Manuscript Accepted: March 25, 2014
Published: April 3, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Pierre Bon, Sherazade Aknoun, Serge Monneret, and Benoit Wattellier, "Enhanced 3D spatial resolution in quantitative phase microscopy using spatially incoherent illumination," Opt. Express 22, 8654-8671 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-7-8654


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