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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 8 — Apr. 21, 2014
  • pp: 9159–9170

Shot-noise limited localization of single 20 nm gold particles with nanometer spatial precision within microseconds

Ying-Hsiu Lin, Wei-Lin Chang, and Chia-Lung Hsieh  »View Author Affiliations

Optics Express, Vol. 22, Issue 8, pp. 9159-9170 (2014)

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Single-particle tracking (SPT) is a powerful approach to investigate dynamics without ensemble average. Continuing effort has been made to track smaller particles with better spatial precision at higher speed. In this work, we demonstrate SPT of 20 nm gold nanoparticle (GNP) with 2 nm spatial precision up to 500 kHz by using microsecond interferometric scattering (μs-iSCAT) microscopy. The linear scattering signal from single GNPs is detected by a high-speed CMOS camera via interference. Through this homodyne detection, shot-noise limited sensitivity, and therefore optimal localization precision are achieved at high speed where considerable electronic noise is present. Using μs-iSCAT microscopy, we observe anomalous diffusion of GNPs labeled to lipid molecules in a supported bilayer membrane prepared on a glass substrate. The combination of nanometer spatial precision and microsecond temporal resolution provides the opportunity to study rapid motions of nano-objects on molecular scale with unprecedented clarity.

© 2014 Optical Society of America

OCIS Codes
(030.4280) Coherence and statistical optics : Noise in imaging systems
(040.1880) Detectors : Detection
(110.0180) Imaging systems : Microscopy
(180.0180) Microscopy : Microscopy
(290.0290) Scattering : Scattering
(290.5850) Scattering : Scattering, particles

ToC Category:

Original Manuscript: January 30, 2014
Revised Manuscript: March 28, 2014
Manuscript Accepted: March 28, 2014
Published: April 8, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Ying-Hsiu Lin, Wei-Lin Chang, and Chia-Lung Hsieh, "Shot-noise limited localization of single 20 nm gold particles with nanometer spatial precision within microseconds," Opt. Express 22, 9159-9170 (2014)

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