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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 8 — Apr. 21, 2014
  • pp: 9368–9379

Laser Doppler holographic microscopy in transmission: application to fish embryo imaging

Nicolas Verrier, Daniel Alexandre, and Michel Gross  »View Author Affiliations

Optics Express, Vol. 22, Issue 8, pp. 9368-9379 (2014)

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We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and the combination of frames used to calculate the hologram, multimodal imaging has been performed. Doppler images of the blood vessels for different Doppler shifts, images where the flow direction is coded in RGB colors or movies showing blood cells individual motion have been obtained as well. The ability to select the Fourier space zone that is used to calculate the signal, makes the method quantitative.

© 2014 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.3340) Medical optics and biotechnology : Laser Doppler velocimetry
(180.3170) Microscopy : Interference microscopy
(290.5850) Scattering : Scattering, particles
(300.6310) Spectroscopy : Spectroscopy, heterodyne
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: February 19, 2014
Revised Manuscript: March 28, 2014
Manuscript Accepted: March 28, 2014
Published: April 10, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Nicolas Verrier, Daniel Alexandre, and Michel Gross, "Laser Doppler holographic microscopy in transmission: application to fish embryo imaging," Opt. Express 22, 9368-9379 (2014)

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