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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 8 — Apr. 21, 2014
  • pp: 9854–9870

A method for super-resolved CARS microscopy with structured illumination in two dimensions

Joo Hyun Park, Sang-Won Lee, Eun Seong Lee, and Jae Yong Lee  »View Author Affiliations


Optics Express, Vol. 22, Issue 8, pp. 9854-9870 (2014)
http://dx.doi.org/10.1364/OE.22.009854


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Abstract

We propose a structured illumination scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy with a resolution surpassing the diffraction limit in two dimensions (2D). By acquiring a set of coherent images of a sample with third-order nonlinear susceptibility illuminated by the phase-matched excitation field of square lattice patterns, a 2D super-resolution CARS image can be reconstructed. We derive a theoretical framework to describe the coherent image formation and reconstruction scheme for this structured illumination CARS imaging system and carry out numerical simulations to investigate its imaging performance. The results demonstrate that our method promises a particular benefit on CARS microscopy by adding the super-resolution capability to improve its 2D spatial resolution by a factor of approximately three.

© 2014 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.2990) Imaging systems : Image formation theory
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 24, 2014
Manuscript Accepted: April 6, 2014
Published: April 16, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Joo Hyun Park, Sang-Won Lee, Eun Seong Lee, and Jae Yong Lee, "A method for super-resolved CARS microscopy with structured illumination in two dimensions," Opt. Express 22, 9854-9870 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-8-9854


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