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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 9 — May. 5, 2014
  • pp: 11001–11010

Axial standing-wave illumination frequency-domain imaging (SWIF)

Benjamin Judkewitz and Changhuei Yang  »View Author Affiliations

Optics Express, Vol. 22, Issue 9, pp. 11001-11010 (2014)

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Despite their tremendous contribution to biomedical research and diagnosis, conventional spatial sampling techniques such as wide-field, point scanning or selective plane illumination microscopy face inherent limiting trade-offs between spatial resolution, field-of-view, phototoxicity and recording speed. Several of these trade-offs are the result of spatial sampling with diffracting beams. Here, we introduce a new strategy for fluorescence imaging, SWIF, which instead encodes the axial profile of a sample in the Fourier domain. We demonstrate how this can be achieved with propagation-invariant illumination patterns that extend over several millimeters and robustly propagate through layers of varying refractive index. This enabled us to image a lateral field-of-view of 0.8 mm x 1.5 mm with an axial resolution of 2.4 µm – greatly exceeding the lateral field-of-view of conventional illumination techniques (~100 µm) at comparable resolution. Thus, SWIF allowed us to surpass the limitations of diffracting illumination beams and untangle lateral field-of-view from resolution.

© 2014 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(110.2945) Imaging systems : Illumination design

ToC Category:

Original Manuscript: March 21, 2014
Revised Manuscript: April 22, 2014
Manuscript Accepted: April 23, 2014
Published: April 30, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Benjamin Judkewitz and Changhuei Yang, "Axial standing-wave illumination frequency-domain imaging (SWIF)," Opt. Express 22, 11001-11010 (2014)

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  1. M. Minsky, “Microscopy Apparatus,” U.S. patent US 3013467 A (December 19, 1961).
  2. B. Bailey, D. L. Farkas, D. L. Taylor, F. Lanni, “Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation,” Nature 366(6450), 44–48 (1993). [CrossRef] [PubMed]
  3. H. Siedentopf, R. Zsigmondy, “Über Sichtbarmachung und Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf Goldrubingläser,” Annalen der Physik 315(1), 1–39 (1902). [CrossRef]
  4. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
  5. H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007). [CrossRef] [PubMed]
  6. P. J. Keller, A. D. Schmidt, J. Wittbrodt, E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008). [CrossRef] [PubMed]
  7. J. Huisken, D. Y. R. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136(12), 1963–1975 (2009). [CrossRef] [PubMed]
  8. J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods 8(10), 811–819 (2011). [CrossRef] [PubMed]
  9. J. Palero, S. I. C. O. Santos, D. Artigas, P. Loza-Alvarez, “A simple scanless two-photon fluorescence microscope using selective plane illumination,” Opt. Express 18(8), 8491–8498 (2010). [CrossRef] [PubMed]
  10. T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8(9), 757–760 (2011). [CrossRef] [PubMed]
  11. J. Huisken, D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007). [CrossRef] [PubMed]
  12. U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012). [CrossRef] [PubMed]
  13. R. Tomer, K. Khairy, F. Amat, P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9(7), 755–763 (2012). [CrossRef] [PubMed]
  14. R. M. Herman, T. A. Wiggins, “Production and uses of diffractionless beams,” JOSA A 8(6), 932–942 (1991). [CrossRef]
  15. F. O. Fahrbach, A. Rohrbach, “Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media,” Nat Commun 3, 632 (2012). [CrossRef] [PubMed]
  16. F. O. Fahrbach, P. Simon, A. Rohrbach, “Microscopy with self-reconstructing beams,” Nat. Photonics 4(11), 780–785 (2010). [CrossRef]
  17. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011). [CrossRef] [PubMed]
  18. L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012). [CrossRef] [PubMed]
  19. D. Feldkhun, K. H. Wagner, “Doppler encoded excitation pattern tomographic optical microscopy,” Appl. Opt. 49(34), H47–H63 (2010). [CrossRef] [PubMed]
  20. A. F. Fercher, C. K. Hitzenberger, G. Kamp, S. Y. El-Zaiat, “Measurement of intraocular distances by backscattering spectral interferometry,” Opt. Commun. 117(1-2), 43–48 (1995). [CrossRef]
  21. M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, A. F. Fercher, “In vivo human retinal imaging by Fourier domain optical coherence tomography,” J. Biomed. Opt. 7(3), 457–463 (2002). [CrossRef] [PubMed]
  22. M. Choma, M. Sarunic, C. Yang, J. Izatt, “Sensitivity advantage of swept source and Fourier domain optical coherence tomography,” Opt. Express 11(18), 2183–2189 (2003). [CrossRef] [PubMed]
  23. E. R. Dowski, W. T. Cathey, “Extended depth of field through wave-front coding,” Appl. Opt. 34(11), 1859–1866 (1995). [CrossRef] [PubMed]
  24. W. T. Cathey, E. R. Dowski, “New paradigm for imaging systems,” Appl. Opt. 41(29), 6080–6092 (2002). [CrossRef] [PubMed]
  25. M. Levoy, R. Ng, A. Adams, M. Footer, M. Horowitz, “Light field microscopy,” Acm T Graphic 25(3), 924–934 (2006). [CrossRef]

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