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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 9 — May. 5, 2014
  • pp: 11043–11060

Direct observation of light focusing by single photoreceptor cell nuclei

Zuzanna Błaszczak, Moritz Kreysing, and Jochen Guck  »View Author Affiliations


Optics Express, Vol. 22, Issue 9, pp. 11043-11060 (2014)
http://dx.doi.org/10.1364/OE.22.011043


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Abstract

The vertebrate retina is inverted with respect to its optical function, which requires light to pass through the entire tissue prior to detection. The last significant barrier for photons to overcome is the outer nuclear layer formed by photoreceptor cell (PRC) nuclei. Here we experimentally characterise the optical properties of PRC nuclei using bright-field defocusing microscopy to capture near-field intensity distributions behind individual nuclei. We find that some nuclei efficiently focus incident light confirming earlier predictions based on comparative studies of chromatin organisation in nocturnal and diurnal mammals. The emergence of light focusing during the development of mouse nuclei highlights the acquired nature of the observed lens-like behaviour. Optical characterisation of these nuclei is an important first step towards an improved understanding of how light transmission through the retina is influenced by its constituents.

© 2014 Optical Society of America

OCIS Codes
(330.0330) Vision, color, and visual optics : Vision, color, and visual optics
(330.5370) Vision, color, and visual optics : Physiological optics
(330.4595) Vision, color, and visual optics : Optical effects on vision
(330.4875) Vision, color, and visual optics : Optics of physiological systems
(330.7331) Vision, color, and visual optics : Visual optics, receptor optics

ToC Category:
Vision, Color, and Visual Optics

History
Original Manuscript: January 2, 2014
Revised Manuscript: April 4, 2014
Manuscript Accepted: April 7, 2014
Published: May 1, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Zuzanna Błaszczak, Moritz Kreysing, and Jochen Guck, "Direct observation of light focusing by single photoreceptor cell nuclei," Opt. Express 22, 11043-11060 (2014)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-22-9-11043


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