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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 3, Iss. 9 — Oct. 26, 1998
  • pp: 315–324

Third harmonic generation microscopy

Jeff A. Squier, Michiel Müller, G. J. Brakenhoff, and Kent R. Wilson  »View Author Affiliations


Optics Express, Vol. 3, Issue 9, pp. 315-324 (1998)
http://dx.doi.org/10.1364/OE.3.000315


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Abstract

Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 μm results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.

© Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(320.7160) Ultrafast optics : Ultrafast technology

ToC Category:
Focus Issue: New trends in biomedical microscopy

History
Original Manuscript: July 24, 1998
Published: October 26, 1998

Citation
Jeffrey Squier, Michiel Muller, G. Brakenhoff, and Kent R. Wilson, "Third harmonic generation microscopy," Opt. Express 3, 315-324 (1998)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-3-9-315


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References

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  2. R. W. Boyd, Nonlinear Optics. (Academic Press, Boston, 1992).
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  4. M. M?ller, J. Squier, K. R. Wilson and G. J. Brakenhoff, "3D-microscopy of transparent objects using third-harmonic generation," J. Microsc. 191, 266-274 (1998). [CrossRef] [PubMed]
  5. J. Squier, M. M?ller, K. R. Wilson, G. J. Brakenhoff, "3D-Microscopy using third harmonic generation at interfaces in biological and nonbiological specimens," to appear in proceedings of Ultrafast Phenomena XI, Garmisch-PartenKirchen/Germany, July 12-17, 1998.
  6. T. Tsang, "Reflected optical harmonics from dielectric mirrors," Appl. Opt., 33, 7720-7723, (1994). [CrossRef] [PubMed]
  7. A. Sievers, B. Buchen, D. Hodick, "Gravity sensing in tip-growing cells," Trends in Plant Sci. 1, 273-279 (1996). [CrossRef]
  8. M. Müller, J. Squier, R. Wolleschensky, U. Simon, G. J. Brakenhoff, "Dispersion pre-compensation of 15 femtosecond optical pulses for high-numerical-aperture objectives," J. Microsc. 191, 141-150 (1998). [CrossRef] [PubMed]

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Supplementary Material


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