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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 3, Iss. 9 — Oct. 26, 1998
  • pp: 315–324

Third harmonic generation microscopy

Jeff A. Squier, Michiel Müller, G. J. Brakenhoff, and Kent R. Wilson  »View Author Affiliations

Optics Express, Vol. 3, Issue 9, pp. 315-324 (1998)

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Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 μm results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.

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OCIS Codes
(110.0110) Imaging systems : Imaging systems
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(320.7160) Ultrafast optics : Ultrafast technology

ToC Category:
Focus Issue: New trends in biomedical microscopy

Original Manuscript: July 24, 1998
Published: October 26, 1998

Jeffrey Squier, Michiel Muller, G. Brakenhoff, and Kent R. Wilson, "Third harmonic generation microscopy," Opt. Express 3, 315-324 (1998)

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