Third harmonic generation microscopy
Optics Express, Vol. 3, Issue 9, pp. 315-324 (1998)
http://dx.doi.org/10.1364/OE.3.000315
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Abstract
Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 μm results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.
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OCIS Codes
(110.0110) Imaging systems : Imaging systems
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(320.7160) Ultrafast optics : Ultrafast technology
ToC Category:
Focus Issue: New trends in biomedical microscopy
History
Original Manuscript: July 24, 1998
Published: October 26, 1998
Citation
Jeffrey Squier, Michiel Muller, G. Brakenhoff, and Kent R. Wilson, "Third harmonic generation microscopy," Opt. Express 3, 315-324 (1998)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-3-9-315
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References
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