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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 3, Iss. 9 — Oct. 26, 1998
  • pp: 332–338

Three-dimensional microscopic tomographic imaging of the cataract in a human lens in vivo

B. R. Masters  »View Author Affiliations


Optics Express, Vol. 3, Issue 9, pp. 332-338 (1998)
http://dx.doi.org/10.1364/OE.3.000332


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Abstract

The problem of three-dimensional visualization of a human lens in vivo has been solved by a technique of volume rendering a transformed series of 60 rotated Scheimpflug (a dual slit reflected light microscope) digital images. The data set was obtained by rotating the Scheimpflug camera about the optic axis of the lens in 3 degree increments. The transformed set of optical sections were first aligned to correct for small eye movements, and then rendered into a volume reconstruction with volume rendering computer graphics techniques. To help visualize the distribution of lens opacities (cataracts) in the living, human lens the intensity of light scattering was pseudocolor coded and the cataract opacities were displayed as a movie.

© Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Focus Issue: New trends in biomedical microscopy

History
Original Manuscript: September 22, 1998
Published: October 26, 1998

Citation
Barry Masters, "Three-dimensional microscopic tomographic imagings of the cataract in a human lens in vivo," Opt. Express 3, 332-338 (1998)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-3-9-332


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References

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  16. Y. Emori, Y. Yasuda, H. Fukuda, K. Sasaki, "Application of image processing technique for analyzing the optical system of the eye," in Advances in Diagnostic Visual Optics, A. Fiorentini, D. L. Guyton, I. M. Siegel, eds. (Springer-Verlag, Berlin, 1987), 55-60.
  17. Y. Sakamoto, K. Sasaki, "Computed tomographic images and three dimensional expression of crystalline lens findings from multiple slices of Scheimpflug slit images," Ophthalmic Res 27 (suppl 1), 94-99 (1995). [CrossRef]
  18. M. Levoy, "Display of Surfaces from Volume Data," PhD dissertation, (University of North Carolina, Chapel Hill, 1989).
  19. B. R. Masters, K. Sasaki, Y. Sakamoto, M. Kojima, Y. Emori, S. Senft, M. Foster, "Three-dimensional volume visualization of the in vivo human ocular lens showing localization of the cataract," Ophthalmic Res, 28(2), 120-126 (1996). [CrossRef]
  20. B. R. Masters, S. L. Senft, "Transformation of a set of slices rotated on a common axis to a set of z-slices: application to three-dimensional visualization of the in vivo human lens," Comput. Med. Imag. Graph. 21(3), 145-151 (1997). [CrossRef]
  21. B. R. Masters, "Optical tomography of the in vivo human lens: three-dimensional visualization of cataracts," J. Biomed. Optics, 1(3), 289-295 (1997).
  22. B. R. Masters, "Three-dimensional visualization of human cataract in vivo," German J Ophthalmol, 5(6), 532- 536 (1997).

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