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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 3, Iss. 9 — Oct. 26, 1998
  • pp: 339–350

Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures

Peter T. C. So, Hyun Kim, and Irene E. Kochevar  »View Author Affiliations


Optics Express, Vol. 3, Issue 9, pp. 339-350 (1998)
http://dx.doi.org/10.1364/OE.3.000339


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Abstract

The non-invasive determination of deep tissue three dimensional structure and biochemistry is the ultimate goal of optical biopsy. Two-photon microscopy has been shown to be a particularly promising approach. The use of infrared radiation in two-photon microscopy is critical for deep tissue imaging since tissue absorption and scattering coefficients for infrared light are much lower than for shorter wavelengths. Equally important, tissue photodamage is localized to the focal region where fluorescence excitation occurs. This report demonstrates that, by means of high resolution two-photon microscopy, skin and subcutaneous tissue structures can be imaged utilizing their endogenous fluorescence. From a freshly prepared tissue punch of a mouse ear, we were able to resolve in 3D both the living and cornified keratinocytes in the epidermis, the collagen/elastin fibers in the dermal layer and the cartilage in the subcutaneous layer. The ability to non-invasively acquire 3D structures of these tissue components may find application in areas such as non-invasive diagnosis of skin cancer and the study of wound healing processes.

© Optical Society of America

OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Focus Issue: New trends in biomedical microscopy

History
Original Manuscript: September 18, 1998
Published: October 26, 1998

Citation
Peter So, Hyun Kim, and Irene Kochevar, "Two-Photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures," Opt. Express 3, 339-350 (1998)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-3-9-339


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