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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 5, Iss. 13 — Dec. 20, 1999
  • pp: 302–317

Reflectance spectroscopy with polarized light: is it sensitive to cellular and nuclear morphology

Konstantin Sokolov, Rebekah Drezek, Kirk Gossage, and Rebecca Richards-Kortum  »View Author Affiliations

Optics Express, Vol. 5, Issue 13, pp. 302-317 (1999)

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We present a method for selective detection of size-dependent scattering characteristics of epithelial cells in vivo based on polarized illumination and polarization sensitive detection of scattered light. We illustrate the method using phantoms designed to simulate squamous epithelial tissue and progressing to epithelial tissue in vitro and in vivo. Elastic light scattering spectroscopy with polarized illumination/detection dramatically reduces background signals due to both diffuse stromal scattering and hemoglobin absorption. Resulting spectra can be described as a linear combination of forward and backscattering components determined from Mie theory. Nuclear sizes and refractive indices extracted by fitting experimental spectra to this model agree well with previous measurements. Reflectance spectroscopy with polarized light can provide quantitative morphological information which could potentially be used for non-invasive detection of neoplastic changes.

© Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.0290) Scattering : Scattering

ToC Category:
Research Papers

Original Manuscript: November 10, 1999
Published: December 20, 1999

Konstantin Sokolov, Rebekah Drezek, Kirk Gossage, and Rebecca Richards-Kortum, "Reflectance spectroscopy with polarized light: is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999)

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