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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 5, Iss. 8 — Oct. 11, 1999
  • pp: 169–175

Laser scanning third-harmonic-generation microscopy in biology

Dvir Yelin and Yaron Silberberg  »View Author Affiliations


Optics Express, Vol. 5, Issue 8, pp. 169-175 (1999)
http://dx.doi.org/10.1364/OE.5.000169


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Abstract

A laser scanning microscope using third-harmonic generation as a probe is shown to produce high-resolution images of transparent biological specimens. Third harmonic light is generated by a tightly focused short-pulse laser beam and collected point-by-point to form a digital image. Demonstrations with two biological samples are presented. Live neurons in a cell culture are imaged with clear and detailed images, including organelles at the threshold of optical resolution. Internal organelles of yeast cells are also imaged, demonstrating the ability of the technique for cellular and intracellular imaging.

© Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Research Papers

History
Original Manuscript: September 28, 1999
Published: October 11, 1999

Citation
Dvir Yelin and Yaron Silberberg, "Laser scanning third-harmonic-generation microscopy in biology," Opt. Express 5, 169-175 (1999)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-5-8-169


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