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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 6, Iss. 7 — Mar. 27, 2000
  • pp: 136–146

An optical coherence microscope for 3-dimensional imaging in developmental biology

Barbara M. Hoeling, Andrew D. Fernandez, Richard C. Haskell, Eric Huang, Whittier R. Myers, Daniel C. Petersen, Sharon E. Ungersma, Ruye Wang, Mary E. Williams, and Scott E. Fraser  »View Author Affiliations


Optics Express, Vol. 6, Issue 7, pp. 136-146 (2000)
http://dx.doi.org/10.1364/OE.6.000136


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Abstract

An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 µm (FWHM), and the depth resolution is 10 µm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.

© Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Research Papers

History
Original Manuscript: February 7, 2000
Published: March 27, 2000

Citation
Barbara Hoeling, Andrew Fernandez, Richard Haskell, Eric Huang, Whittier Myers, Daniel Petersen, Sharon Ungersma, Ruye Wang, Mary Williams, and Scott Fraser, "An optical coherence microscope for 3-dimensional imaging in developmental biology," Opt. Express 6, 136-146 (2000)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-6-7-136


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