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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 7, Iss. 10 — Nov. 6, 2000
  • pp: 336–341

Confocal microscopy using an InGaN violet laser diode at 406nm

J. M. Girkin, A. I. Ferguson, D. L. Wokosin, and A. M. Gurney  »View Author Affiliations


Optics Express, Vol. 7, Issue 10, pp. 336-341 (2000)
http://dx.doi.org/10.1364/OE.7.000336


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Abstract

We report on the application of a novel all-solid-state violet laser diode source to confocal microscopy. The source has the potential to replace argon ion lasers in a range of fluorescence based imaging systems. Improvements in system performance and image quality through the use of anamorphic prisms to modify the beam profile have been characterised. These modifications have permitted high quality, optically sectioned images to be obtained from laser diodes operating around 406nm. Living mammalian cells stained with a range of biologically significant fluorophores have been imaged. In addition, it has been shown that at this wavelength it is possible to image dyes that normally require excitation with UV argon laser lines.

© Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy

ToC Category:
Research Papers

History
Original Manuscript: October 3, 2000
Published: November 6, 2000

Citation
John Girkin, Allister Ferguson, David Wokosin, and A. Gurney, "Confocal microscopy using an InGaN violet laser diode at 406nm," Opt. Express 7, 336-341 (2000)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-7-10-336


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References

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