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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 8, Iss. 6 — Mar. 12, 2001
  • pp: 335–343

Low temperature fluorescence imaging of freeze-trapped human cervical tissues

Nirmala Ramanujam, Rebecca Richards-Kortum, Sharon Thomsen, Anita Mahadevan-Jansen, Michele Follen, and Britton Chance  »View Author Affiliations


Optics Express, Vol. 8, Issue 6, pp. 335-343 (2001)
http://dx.doi.org/10.1364/OE.8.000335


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Abstract

We characterized the fluorescence intensity distribution within the epithelia and stroma of frozen human cervical tissues at the following excitation-emission wavelength pairs: 440, 525 nm and 365, 460 nm. The intensities at both excitation-emission wavelength pairs are significantly lower in the epithelia of severely dysplastic tissues, relative to that in normal and inflammatory tissues. Furthermore, there are small differences in (1) the epithelial intensity of severe dysplasia and mild dysplasia at 440, 525 nm and (2) the stromal intensity of inflammatory and severely dysplastic tissues at 365, 460 nm. A comparison of the ratio of intensities at 440, 525 nm and 365, 460 nm between the epithelia of each tissue type indicates that this ratio is lowest in severely dysplastic tissues. It is interesting to note that the epithelial and stromal intensities are comparable at 365, 460 nm; however, at 440, 525 nm, the epithelial intensity is more than a factor of two less that that of the stroma for all tissue types.

© Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

History
Original Manuscript: January 2, 2001
Published: March 12, 2001

Citation
Nirmala Ramanujam, Rebecca Richards-Kortum, Sharon Thomsen, Anita Mahadevan-Jansen, Michele Follen, and Britton Chance, "Low Temperature Fluorescence Imaging of Freeze-trapped Human Cervical Tissues," Opt. Express 8, 335-343 (2001)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-8-6-335


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