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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 9, Iss. 12 — Dec. 3, 2001
  • pp: 637–644

Confocal microscopy with a high numerical aperture parabolic mirror

A. Drechsler, M. A. Lieb, C. Debus, A. J. Meixner, and G. Tarrach  »View Author Affiliations


Optics Express, Vol. 9, Issue 12, pp. 637-644 (2001)
http://dx.doi.org/10.1364/OE.9.000637


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Abstract

A novel high-resolution stage scanning confocal microscope for fluorescence microscopy and spatially resolved spectroscopy with a high numerical aperture (NA≈1) parabolic mirror objective is investigated. A spatial resolution close to the diffraction limit is achieved. As microscopic fluorescent test objects, dye-loaded zeolite microcrystals (diameter approx. 0.4 µm) and single fluorescent molecules were used. Confocal fluorescence images show a spatial resolution of Δx=0.8·λ both at room temperature and at 1.8 K. Imaging of a quasi-point light source and focusing by the parabolic mirror were investigated experimentally and theoretically. Deviations between the theoretical results for a perfect parabolic mirror and the experimental results can be attributed to small deviations of the mirror profile from an ideal parabola.

© Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(230.4040) Optical devices : Mirrors

ToC Category:
Research Papers

History
Original Manuscript: November 6, 2001
Published: December 3, 2001

Citation
A. Drechsler, M. Lieb, C. Debus, Alfred Meixner, and G. Tarrach, "Confocal microscopy with a high numerical aperture parabolic mirror," Opt. Express 9, 637-644 (2001)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-9-12-637


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