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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 9, Iss. 13 — Dec. 17, 2001
  • pp: 802–812

Analysis of nonlinear relation for skin hemoglobin imaging

Manami Kobayashi, Yasunobu Ito, Naofumi Sakauchi, Ichiro Oda, Ikuo Konishi, and Yoshio Tsunazawa  »View Author Affiliations

Optics Express, Vol. 9, Issue 13, pp. 802-812 (2001)

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This paper discusses the accuracy of the optical determination of the oxygenated and deoxygenated hemoglobin content of human skin under the influence of a melanin layer for a multi-wavelengths imager. The relation between the nonlinear results by Monte Carlo simulation (MCS) and the modified Lambert Beer’s law (MLB) is also clarified, emphasizing the importance of the absolute values of skin pigments and their influence on the mean path-length used in MLB. The fitting procedure of the MCS data to the actual skin spectra is shown to obtain the absolute values. It is also shown that once the proper mean path-lengths have been determined, MLB can be used fairly well within an accuracy of 80% compared with MCS. Images of oxygenated hemoglobin with a newly-developed four-wavelength camera are presented to demonstrate the advantages of a multi-wavelength system.

© Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Research Papers

Original Manuscript: October 23, 2001
Published: December 17, 2001

Manami Kobayashi, Yasunobu Ito, Naofumi Sakauchi, Ichiro Oda, Ikuo Konishi, and Yoshio Tsunazawa, "Analysis of nonlinear relation for skin hemoglobin imaging," Opt. Express 9, 802-812 (2001)

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