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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 9, Iss. 13 — Dec. 17, 2001
  • pp: 821–830

Fiber confocal reflectance microscope (FCRM) for in-vivo imaging

Chen Liang, Michael R. Descour, Kung-Bin Sung, and Rebecca Richards-Kortum  »View Author Affiliations

Optics Express, Vol. 9, Issue 13, pp. 821-830 (2001)

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In-vivo imaging can be achieved with a coherent-fiber-bundle based confocal reflectance microscope. Such a microscope could provide the means to detect pre-cancerous lesions in the cervix by characterizing cells’ nuclear-to-cytoplasmic ratio. In this paper we present the design of such a fiber confocal reflectance microscope, with an emphasis on its optical sub-systems. The optical sub-systems consist of a commercially available microscope objective and custom designed telescope, scan lens, and coupling lens systems. The performance of the fiber confocal reflectance microscope was evaluated by imaging a resolution bar target and human cervical biopsy tissues. The results presented in this paper demonstrate a lateral resolution of 2 µm and axial resolution of 6 µm. The sensitivity of the system defined by the smallest refractive-index mismatch that can be detected is approximately Δn~0.05.

© Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.1790) Medical optics and biotechnology : Confocal microscopy

ToC Category:
Research Papers

Original Manuscript: October 26, 2001
Published: December 17, 2001

Chen Liang, Michael Descour, Kung-Bin Sung, and Rebecca Richards-Kortum, "Fiber confocal reflectance microscope (FCRM) for in-vivo imaging," Opt. Express 9, 821-830 (2001)

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