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Nanoscale imaging using deep ultraviolet digital holographic microscopy |
Optics Express, Vol. 18, Issue 13, pp. 14159-14164 (2010)
http://dx.doi.org/10.1364/OE.18.014159
Acrobat PDF (1818 KB)
Abstract
A deep ultraviolet off-axis digital holographic microscope (DHM) is presented. The microscope has been arranged with as least as possible optical elements in the imaging path to avoid aberration due to the non-perfect optical elements. A high resolution approach has been implemented in the setup using oblique illumination to overcome the limitation introduced by the optical system. To examine the resolution of the system a nano-structured template has been designed and the result confirms the submicron and nanoscale resolution of the arranged DHM setup.
© 2010 OSA
1. Introduction
M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006). [CrossRef] [PubMed]
S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett. 19(11), 780–782 (1994). [CrossRef] [PubMed]
K. Carlsson, P. E. Danielsson, R. Lenz, A. Liljeborg, L. Majlöf, and N. Aslund, “Three-dimensional microscopy using a confocal laser scanning microscope,” Opt. Lett. 10(2), 53–55 (1985). [CrossRef] [PubMed]
J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
D. Gabor, “A new microscopic principle,” Nature 161(4098), 777–778 (1948). [CrossRef] [PubMed]
J. W. Goodman and R. W. Lawrence, “Digital image formation from electronically detected holograms,” Appl. Phys. Lett. 11(3), 77–79 (1967). [CrossRef]
I. Yamaguchi and T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22(16), 1268–1270 (1997). [CrossRef] [PubMed]
G. Pedrini, F. Zhang, and W. Osten, “Digital holographic microscopy in the deep (193 nm) ultraviolet,” Appl. Opt. 46(32), 7829–7835 (2007). [CrossRef] [PubMed]
P. Ferraro, G. Coppola, S. De Nicola, A. Finizio, and G. Pierattini, “Digital holographic microscope with automatic focus tracking by detecting sample displacement in real time,” Opt. Lett. 28(14), 1257–1259 (2003). [CrossRef] [PubMed]
I. Yamaguchi, T. Ida, and M. Yokota, “Measurement of Surface Shape and Position by Phase-Shifting Digital Holography,” Strain 44(5), 349–356 (2008). [CrossRef]
L. Martínez-León and B. Javidi, “Synthetic aperture single-exposure on-axis digital holography,” Opt. Express 16(1), 161–169 (2008). [CrossRef] [PubMed]
S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006). [CrossRef] [PubMed]
C. Liu, Z. Liu, F. Bo, Y. Wang, and J. Zhu, “Super-resolution digital holographic imaging method,” Appl. Phys. Lett. 81(17), 3143–3145 (2002). [CrossRef]
M. Paturzo, F. Merola, S. Grilli, S. De Nicola, A. Finizio, and P. Ferraro, “Super-resolution in digital holography by a two-dimensional dynamic phase grating,” Opt. Express 16(21), 17107–17118 (2008). [CrossRef] [PubMed]
V. Micó, Z. Zalevsky, C. Ferreira, and J. García, “Superresolution digital holographic microscopy for three-dimensional samples,” Opt. Express 16(23), 19260–19270 (2008). [CrossRef]
S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006). [CrossRef] [PubMed]
2. Experiment
2.1. Off-axis digital holography setup
2.2. Deep-UV-objective
2.3. Oblique illumination approach
2.4. Nano-structured template
3. Result
4. Conclusion
Acknowledgment
References and links
M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006). [CrossRef] [PubMed] | |
S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett. 19(11), 780–782 (1994). [CrossRef] [PubMed] | |
K. Carlsson, P. E. Danielsson, R. Lenz, A. Liljeborg, L. Majlöf, and N. Aslund, “Three-dimensional microscopy using a confocal laser scanning microscope,” Opt. Lett. 10(2), 53–55 (1985). [CrossRef] [PubMed] | |
J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed] | |
D. Gabor, “A new microscopic principle,” Nature 161(4098), 777–778 (1948). [CrossRef] [PubMed] | |
J. W. Goodman and R. W. Lawrence, “Digital image formation from electronically detected holograms,” Appl. Phys. Lett. 11(3), 77–79 (1967). [CrossRef] | |
L. Yaroslavsky, Digital Holography and Digital Image Processing: Principles, Methods, Algorithms, (Kluwer Academic Publishers, 2004). | |
I. Yamaguchi and T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22(16), 1268–1270 (1997). [CrossRef] [PubMed] | |
B. Javidi and E. Tajahuerce, “Three-dimensional object recognition by use of digital holography,” Opt. Lett. 25(9), 610–612 (2000). [CrossRef] | |
P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005). [CrossRef] [PubMed] | |
L. Martínez-León, G. Pedrini, and W. Osten, “Applications of short-coherence digital holography in microscopy,” Appl. Opt. 44(19), 3977–3984 (2005). [CrossRef] [PubMed] | |
F. Zhang, G. Pedrini, and W. Osten, “Reconstruction algorithm for high-numerical-aperture holograms with diffraction-limited resolution,” Opt. Lett. 31(11), 1633–1635 (2006). [CrossRef] [PubMed] | |
G. Pedrini, F. Zhang, and W. Osten, “Digital holographic microscopy in the deep (193 nm) ultraviolet,” Appl. Opt. 46(32), 7829–7835 (2007). [CrossRef] [PubMed] | |
P. Ferraro, G. Coppola, S. De Nicola, A. Finizio, and G. Pierattini, “Digital holographic microscope with automatic focus tracking by detecting sample displacement in real time,” Opt. Lett. 28(14), 1257–1259 (2003). [CrossRef] [PubMed] | |
I. Yamaguchi, T. Ida, and M. Yokota, “Measurement of Surface Shape and Position by Phase-Shifting Digital Holography,” Strain 44(5), 349–356 (2008). [CrossRef] | |
L. Martínez-León and B. Javidi, “Synthetic aperture single-exposure on-axis digital holography,” Opt. Express 16(1), 161–169 (2008). [CrossRef] [PubMed] | |
C. Liu, Z. Liu, F. Bo, Y. Wang, and J. Zhu, “Super-resolution digital holographic imaging method,” Appl. Phys. Lett. 81(17), 3143–3145 (2002). [CrossRef] | |
M. Paturzo, F. Merola, S. Grilli, S. De Nicola, A. Finizio, and P. Ferraro, “Super-resolution in digital holography by a two-dimensional dynamic phase grating,” Opt. Express 16(21), 17107–17118 (2008). [CrossRef] [PubMed] | |
V. Micó, Z. Zalevsky, C. Ferreira, and J. García, “Superresolution digital holographic microscopy for three-dimensional samples,” Opt. Express 16(23), 19260–19270 (2008). [CrossRef] | |
C. Yuan, H. Zhai, and H. Liu, “Angular multiplexing in pulsed digital holography for aperture synthesis,” Opt. Lett. 33(20), 2356–2358 (2008). [CrossRef] [PubMed] | |
S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006). [CrossRef] [PubMed] |
OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography
ToC Category:
Microscopy
History
Original Manuscript: May 17, 2010
Revised Manuscript: June 11, 2010
Manuscript Accepted: June 11, 2010
Published: June 16, 2010
Citation
Ahmad Faridian, David Hopp, Giancarlo Pedrini, Ulrike Eigenthaler, Michael Hirscher, and Wolfgang Osten, "Nanoscale imaging using deep ultraviolet digital holographic microscopy," Opt. Express 18, 14159-14164 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-13-14159
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References
- M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006). [CrossRef] [PubMed]
- S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett. 19(11), 780–782 (1994). [CrossRef] [PubMed]
- K. Carlsson, P. E. Danielsson, R. Lenz, A. Liljeborg, L. Majlöf, and N. Aslund, “Three-dimensional microscopy using a confocal laser scanning microscope,” Opt. Lett. 10(2), 53–55 (1985). [CrossRef] [PubMed]
- J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004). [CrossRef] [PubMed]
- D. Gabor, “A new microscopic principle,” Nature 161(4098), 777–778 (1948). [CrossRef] [PubMed]
- J. W. Goodman and R. W. Lawrence, “Digital image formation from electronically detected holograms,” Appl. Phys. Lett. 11(3), 77–79 (1967). [CrossRef]
- L. Yaroslavsky, Digital Holography and Digital Image Processing: Principles, Methods, Algorithms, (Kluwer Academic Publishers, 2004).
- I. Yamaguchi and T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22(16), 1268–1270 (1997). [CrossRef] [PubMed]
- B. Javidi and E. Tajahuerce, “Three-dimensional object recognition by use of digital holography,” Opt. Lett. 25(9), 610–612 (2000). [CrossRef]
- P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005). [CrossRef] [PubMed]
- L. Martínez-León, G. Pedrini, and W. Osten, “Applications of short-coherence digital holography in microscopy,” Appl. Opt. 44(19), 3977–3984 (2005). [CrossRef] [PubMed]
- F. Zhang, G. Pedrini, and W. Osten, “Reconstruction algorithm for high-numerical-aperture holograms with diffraction-limited resolution,” Opt. Lett. 31(11), 1633–1635 (2006). [CrossRef] [PubMed]
- G. Pedrini, F. Zhang, and W. Osten, “Digital holographic microscopy in the deep (193 nm) ultraviolet,” Appl. Opt. 46(32), 7829–7835 (2007). [CrossRef] [PubMed]
- P. Ferraro, G. Coppola, S. De Nicola, A. Finizio, and G. Pierattini, “Digital holographic microscope with automatic focus tracking by detecting sample displacement in real time,” Opt. Lett. 28(14), 1257–1259 (2003). [CrossRef] [PubMed]
- I. Yamaguchi, T. Ida, and M. Yokota, “Measurement of Surface Shape and Position by Phase-Shifting Digital Holography,” Strain 44(5), 349–356 (2008). [CrossRef]
- L. Martínez-León and B. Javidi, “Synthetic aperture single-exposure on-axis digital holography,” Opt. Express 16(1), 161–169 (2008). [CrossRef] [PubMed]
- C. Liu, Z. Liu, F. Bo, Y. Wang, and J. Zhu, “Super-resolution digital holographic imaging method,” Appl. Phys. Lett. 81(17), 3143–3145 (2002). [CrossRef]
- M. Paturzo, F. Merola, S. Grilli, S. De Nicola, A. Finizio, and P. Ferraro, “Super-resolution in digital holography by a two-dimensional dynamic phase grating,” Opt. Express 16(21), 17107–17118 (2008). [CrossRef] [PubMed]
- V. Micó, Z. Zalevsky, C. Ferreira, and J. García, “Superresolution digital holographic microscopy for three-dimensional samples,” Opt. Express 16(23), 19260–19270 (2008). [CrossRef]
- C. Yuan, H. Zhai, and H. Liu, “Angular multiplexing in pulsed digital holography for aperture synthesis,” Opt. Lett. 33(20), 2356–2358 (2008). [CrossRef] [PubMed]
- S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, “Synthetic aperture fourier holographic optical microscopy,” Phys. Rev. Lett. 97(16), 168102 (2006). [CrossRef] [PubMed]
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