Abstract
We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos.
© 1999 Optical Society of America
Full Article | PDF ArticleMore Like This
Faming Xu, Haridas E. Pudavar, Paras N. Prasad, and David Dickensheets
Opt. Lett. 24(24) 1808-1810 (1999)
Natallia Uzunbajakava and Cees Otto
Opt. Lett. 28(21) 2073-2075 (2003)
A. D. Aguirre, P. Hsiung, T. H. Ko, I. Hartl, and J. G. Fujimoto
Opt. Lett. 28(21) 2064-2066 (2003)