OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 24, Iss. 14 — Jul. 15, 1999
  • pp: 969–971

Combined scanning optical coherence and two-photon-excited fluorescence microscopy

E. Beaurepaire, L. Moreaux, F. Amblard, and J. Mertz  »View Author Affiliations

Optics Letters, Vol. 24, Issue 14, pp. 969-971 (1999)

View Full Text Article

Acrobat PDF (837 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos.

© 1999 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes

E. Beaurepaire, L. Moreaux, F. Amblard, and J. Mertz, "Combined scanning optical coherence and two-photon-excited fluorescence microscopy," Opt. Lett. 24, 969-971 (1999)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
  2. J. M. Schmitt, S. L. Hee, and K. M. Yung, Opt. Commun. 142, 203 (1997).
  3. G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Bopart, C. Pitris, J. F. Southern, and J. G. Fujimoto, Science 276, 2037 (1997).
  4. S. A. Boppart, B. E. Bouma, C. Pitris, J. F. Southern, M. E. Brezinski, and J. G. Fujimoto, Nature Med. 4, 861 (1998).
  5. J. A. Izatt, M. D. Kulkarni, H.-W. Wang, K. Kobayashi, and M. V. Sivak, Jr., IEEE J. Sel. Top. Quantum Electron. 2, 1017 (1996).
  6. A. Gh. Podoleanu, G. M. Dobre, D. J. Webb, and D. A. Jackson, Opt. Lett. 22, 1039 (1997).
  7. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  8. W. Denk, D. Piston, and W. W. Webb, in The Handbook of Confocal Microscopy, 2nd ed., J. Pawley, ed. (Plenum, New York, 1995), p. 445.
  9. M. Kempe, W. Rudolph, and E. Welsch, J. Opt. Soc. Am. A 13, 46 (1996).
  10. E. Beaurepaire, A. C. Boccara, M. Lebec, L. Blanchot, and H. Saint-Jalmes, Opt. Lett. 23, 244 (1998).
  11. M. J. Everett, K. Schoenenberger, B. W. Colston, Jr., and L. B. Da Silva, Opt. Lett. 23, 228 (1998).
  12. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, Opt. Lett. 19, 590 (1994).
  13. J. M. Schmitt and A. Knüttel, J. Opt. Soc. Am. A 14, 1231 (1997).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited