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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 24, Iss. 14 — Jul. 15, 1999
  • pp: 969–971

Combined scanning optical coherence and two-photon-excited fluorescence microscopy

E. Beaurepaire, L. Moreaux, F. Amblard, and J. Mertz  »View Author Affiliations


Optics Letters, Vol. 24, Issue 14, pp. 969-971 (1999)
http://dx.doi.org/10.1364/OL.24.000969


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Abstract

We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos.

© 1999 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes

Citation
E. Beaurepaire, L. Moreaux, F. Amblard, and J. Mertz, "Combined scanning optical coherence and two-photon-excited fluorescence microscopy," Opt. Lett. 24, 969-971 (1999)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-24-14-969

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