Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser
Optics Letters, Vol. 24, Issue 2, pp. 116-118 (1999)
http://dx.doi.org/10.1364/OL.24.000116
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Abstract
Reproducible fluorescence spectra of individual 2- to 5-µm -diameter biological aerosol particles excited with a single shot from a Q -switched laser (266 or 351 nm) have been obtained with highly improved signal-to-noise ratios. Critical to the advance are crossed diode-laser trigger beams, which precisely define the sample volume, and a reflecting objective, which minimizes chromatic aberration and has a large N.A. for collecting fluorescence. Several allergens (red oak, meadow oat pollen, paper mulberry pollen, and puffball spores) have different fluorescence spectra. Bacillus subtilis fluorescence spectrum deteriorates at high 266-nm incident intensity. Dry riboflavin particles illuminated with a 351-nm light exhibit a new 420-nm fluorescence peak that grows nonlinearly with laser pulse energy.
© 1999 Optical Society of America
[Optical Society of America ]
OCIS Codes
(010.1110) Atmospheric and oceanic optics : Aerosols
(140.3610) Lasers and laser optics : Lasers, ultraviolet
(260.2510) Physical optics : Fluorescence
(300.6170) Spectroscopy : Spectra
Citation
Yong-le Pan, Stephen Holler, Richard K. Chang, Steven C. Hill, Ronald G. Pinnick, Stanley Niles, and Jerold R. Bottiger, "Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser," Opt. Lett. 24, 116-118 (1999)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-24-2-116
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