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Optics Letters

Optics Letters


  • Vol. 24, Iss. 23 — Dec. 1, 1999
  • pp: 1723–1725

Integrated optical biosensor for detection of multivalent proteins

Dan Kelly, Karen M. Grace, Xuedong Song, Basil I. Swanson, Daniel Frayer, Sergio B. Mendes, and Nasser Peyghambarian  »View Author Affiliations

Optics Letters, Vol. 24, Issue 23, pp. 1723-1725 (1999)

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We have developed a simple, highly sensitive and specific optical waveguide sensor for the detection of multivalent proteins. The optical biosensor is based on optically tagged glycolipid receptors embedded within a fluid phospholipid bilayer membrane formed upon the surface of a planar optical waveguide. Binding of multivalent cholera toxin triggers a fluorescence resonance energy transfer that results in a two-color optical change that is monitored by measurement of emitted luminescence above the waveguide surface. The sensor approach is highly sensitive and specific and requires no additional reagents and washing steps. Demonstration of protein-receptor recognition by use of planar optical waveguides provides a path forward for the development of fieldable miniaturized biosensor arrays.

© 1999 Optical Society of America

OCIS Codes
(130.0130) Integrated optics : Integrated optics
(130.6010) Integrated optics : Sensors
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

Dan Kelly, Karen M. Grace, Xuedong Song, Basil I. Swanson, Daniel Frayer, Sergio B. Mendes, and Nasser Peyghambarian, "Integrated optical biosensor for detection of multivalent proteins," Opt. Lett. 24, 1723-1725 (1999)

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  1. R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger, and C. A. Mirkin, Science 277, 1078 (1997).
  2. B. D. Hames and S. J. Higgins, eds., Gene Probes (IRL Press, New York, 1995).
  3. J. Wang, E. Palecek, P. E. Nielsen, G. Rivas, X. H. Cai, H. Shiraishi, N. Dontha, D. B. Luo, and P. A. M. Farias, J. Am. Chem. Soc. 118, 7667 (1996).
  4. P. Wood, in Principles and Practice of Immunoassay, C. P. Price and D. J. Newman, eds. (Stockton, New York, 1991), p. 365.
  5. J. R. Astles and W. G. Miller, Anal. Chem. 66, 167 (1994).
  6. S. P. A. Fodor, J. L. Read, M. C. Pirrung, L. Stryer, A. T. Lu, and D. Solas, Science 251, 767 (1991).
  7. B. A. Cornell, V. L. B. Braach-Maksvytis, L. G. King, P. D. Osman, B. Raguse, L. Wieczorek, and R. J. Pace, Nature 387, 580 (1997).
  8. X. Song, J. Nolan, and B. I. Swanson, J. Am. Chem. Soc. 120, 11514 (1998).
  9. J. Dubendorfer, R. E. Kunz, E. Schurmann, G. L. Duveneck, and M. Ehrat, J. Biomed. Opt. 2, 391 (1997).
  10. B. Klee, G. L. Duveneck, P. Oroszlan, M. Ehrat, and H. M. Widmer, Sensors Actuators B 29, 307 (1995).
  11. G. L. Duveneck, M. Pawlak, D. Neuschafer, E. Bar, W. Budach, U. Pieles, and M. Ehrat, Sensors Actuators B 38–39, 88 (1997).
  12. S. J. Choquette, L. Locascio-Brown, and R. A. Durst, Proc. SPIE 1368, 258 (1990).
  13. The isobestic point for BTMR-GM 1 and BTR-GM 1 incorporated within a POPC bilayer was determined in Ref.
  14. X. Song and B. I. Swanson, Anal. Chem. 71, 2097 (1999).

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