OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 24, Iss. 24 — Dec. 15, 1999
  • pp: 1832–1834

Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity

Richard A. Farrer, Michael J. R. Previte, Christopher E. Olson, Lynn Anne Peyser, John T. Fourkas, and Peter T. C. So  »View Author Affiliations


Optics Letters, Vol. 24, Issue 24, pp. 1832-1834 (1999)
http://dx.doi.org/10.1364/OL.24.001832


View Full Text Article

Acrobat PDF (726 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes.

© 1999 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes

Citation
Richard A. Farrer, Michael J. R. Previte, Christopher E. Olson, Lynn Anne Peyser, John T. Fourkas, and Peter T. C. So, "Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity," Opt. Lett. 24, 1832-1834 (1999)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-24-24-1832

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited