We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes.
© 1999 Optical Society of America
Richard A. Farrer, Michael J. R. Previte, Christopher E. Olson, Lynn Anne Peyser, John T. Fourkas, and Peter T. C. So, "Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity," Opt. Lett. 24, 1832-1834 (1999)