Multiple-objective microscopy with three-dimensional resolution near 100 nm and a long working distance
Optics Letters, Vol. 26, Issue 21, pp. 1684-1686 (2001)
http://dx.doi.org/10.1364/OL.26.001684
Acrobat PDF (178 KB)
Abstract
The resolution of microscopes is limited by the sizes of their point-spread functions. The invention of confocal, theta, and 4Pi microscopes has permitted the classic Abbe limit to be exceeded. We propose the use of a combination of 4Pi and theta microscopy to decrease resolution by using four illumination objectives and two detection objectives. Using middle numerical aperture, long-working-distance objectives yielded a resolution near 100 nm in the three dimensions, which opens the possibility of exploring large volumes with a high resolution.
© 2001 Optical Society of America
[Optical Society of America ]
OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
Citation
O. Haeberlé, C. Xu, A. Dieterlen, and S. Jacquey, "Multiple-objective microscopy with three-dimensional resolution near 100 nm and a long working distance," Opt. Lett. 26, 1684-1686 (2001)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-26-21-1684
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Whole-field optically sectioned fluorescence lifetime imaging (OL)
- Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy (JOSAA)
- Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy (OE)
- Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging (OE)
- Reconstruction of objects above and below the objective focal plane with dimensional fidelity by FINCH fluorescence microscopy (OE)
Related Conference Papers
- Fluorescent Nanoscope - experience of transferring science to business
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
« Previous Article | Next Article »
OSA is a member of 