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Optics Letters

Optics Letters


  • Vol. 28, Iss. 14 — Jul. 15, 2003
  • pp: 1221–1223

Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells

Andrew C. Millard, Lei Jin, Aaron Lewis, and Leslie M. Loew  »View Author Affiliations

Optics Letters, Vol. 28, Issue 14, pp. 1221-1223 (2003)

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We report what is to our knowledge the first optical imaging of voltage-clamped cells by second-harmonic generation. For the membrane-staining styryl dye di-4-ANEPPS, we determined the sensitivity of second-harmonic generation to be 18%/100 mV at an excitation wavelength of 850 nm. This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells.

© 2003 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4350) Nonlinear optics : Nonlinear optics at surfaces
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials

Andrew C. Millard, Lei Jin, Aaron Lewis, and Leslie M. Loew, "Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells," Opt. Lett. 28, 1221-1223 (2003)

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