OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 28, Iss. 19 — Oct. 1, 2003
  • pp: 1772–1774

Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy

Chau-Hwang Lee, Hui-Yu Chiang, and Hong-Yao Mong  »View Author Affiliations


Optics Letters, Vol. 28, Issue 19, pp. 1772-1774 (2003)
http://dx.doi.org/10.1364/OL.28.001772


View Full Text Article

Acrobat PDF (501 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Using the nanometer depth sensitivity of differential confocal microscopy, we detect surface features of lateral dimensions smaller than the diffraction limit without fluorescence labeling. The lateral resolution of the topographic images is further enhanced by a maximum-likelihood estimation algorithm. Based on the comparison of signal and noise at high spatial frequency, we estimate the best lateral resolution of the enhanced images to be 0.15λ . In addition, on composite samples this technique can simultaneously display sub-diffraction-limit topographic features and reflectivity heterogeneity.

© 2003 Optical Society of America

OCIS Codes
(100.3020) Image processing : Image reconstruction-restoration
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy

Citation
Chau-Hwang Lee, Hui-Yu Chiang, and Hong-Yao Mong, "Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy," Opt. Lett. 28, 1772-1774 (2003)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-28-19-1772


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. W. A. Carrington, R. M. Lynch, E. D. W. Moore, G. Isenberg, K. E. Fogarty, and F. S. Fay, Science 268, 1483 (1995).
  2. M. Schrader, S. W. Hell, and H. T. M. van der Voort, Appl. Phys. Lett. 69, 3644 (1996).
  3. T. A. Klar and S. W. Hell, Opt. Lett. 24, 954 (1999).
  4. M. Dyba and S. W. Hell, Phys. Rev. Lett. 88, 163901 (2002).
  5. M. G. L. Gustafsson, J. Microsc. 198, 82 (2000).
  6. J. T. Frohn, H. F. Knapp, and A. Stemmer, Proc. Natl. Acad. Sci. USA 97, 7232 (2000).
  7. A. M. van Oijen, J. Köhler, J. Schmidt, M. Müller, and G. J. Brakenhoff, J. Opt. Soc. Am. A 16, 909 (1999).
  8. Q. Zhan and J. R. Leger, Opt. Lett. 27, 821 (2002).
  9. C.-H. Lee and J. Wang, Opt. Commun. 135, 233 (1997).
  10. C.-W. Tsai, C.-H. Lee, and J. Wang, Opt. Lett. 24, 1732 (1999).
  11. See, for example, G. M. P. van Kempen, H. T. M. van der Voort, J. G. J. Bauman, and K. C. Strasters, IEEE Eng. Med. Biol. Mag. 15, 76 (1996).
  12. J.-A. Conchello, J. Opt. Soc. Am. A 15, 2609 (1998).
  13. J. Markham and J.-A. Conchello, J. Opt. Soc. Am. A 18, 1062 (2001).
  14. I. Csiszár, Ann. Stat. 19, 2033 (1991).
  15. D. L. Snyder, M. I. Miller, L. J. Thomas, Jr., and D. G. Politte, IEEE Trans. Med. Imaging 6, 228 (1987).
  16. We thank C.-D. Chen of the Institute of Physics, Academia Sinica, for providing this sample.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited