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Optics Letters

Optics Letters


  • Vol. 29, Iss. 24 — Dec. 15, 2004
  • pp: 2884–2886

Fluorescence-lifetime imaging with a multifocal two-photon microscope

S. Lévêque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges  »View Author Affiliations

Optics Letters, Vol. 29, Issue 24, pp. 2884-2886 (2004)

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Two-photon microscopy is a powerful tool for imaging of cells or tissues. However, it presents the drawback of being a laser-scanning technique that involves a long acquisition time for fluorescence-lifetime imaging. Thus it is commonly limited to intensity images that give only indications of the location of fluorophores but do not identify the physicochemical properties and interactions between cells' components. To protect biological samples from experiments that are too long and to provide a more comprehensive spectroscopic tool we have developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while retaining the possibility of measuring both intensity and lifetime images of the sample.

© 2004 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes

S. Lévêque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges, "Fluorescence-lifetime imaging with a multifocal two-photon microscope," Opt. Lett. 29, 2884-2886 (2004)

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  1. J. Bewersdorf, R. Pick, and S. H. Hell, Opt. Lett. 23, 655 (1998).
  2. D. N. Fittinghoff, P. W. Wiseman, and J. Squier, Opt. Express 7(8), 273 (2000), http://www.opticsexpress.org.
  3. T. Nielsen, M. Fricke, D. Hellweg, and P. Andresen, J. Microsc. 201, 368 (2001).
  4. S. Lévêque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges, Proc. SPIE 5139, 173 (2003).
  5. M. Straub and S. H. Hell, Appl. Phys. Lett. 73, 1769 (1998).
  6. A. H. Buist, M. Muller, J. Squier, and G. J. Brakenhoff, J. Microsc. 192, 2176 (1998).
  7. A. Egner and S. H. Hell, J. Opt. Soc. Am. A 17, 1192 (2000).
  8. S. E. D. Webb, Y. Gu, S. Lévêque-Fort, J. Siegel, M. J. Cole, K. Dowling, R. Jones, P. M. W. French, M. A. A. Neil, R. Jukaitis, L. O. D. Sucharov, T. Wilson, and M. J. Lever, Rev. Sci. Instrum. 73, 1898 (2002).

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