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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 30, Iss. 10 — May. 15, 2005
  • pp: 1162–1164

Spectral-domain phase microscopy

Michael A. Choma, Audrey K. Ellerbee, Changhuei Yang, Tony L. Creazzo, and Joseph A. Izatt  »View Author Affiliations


Optics Letters, Vol. 30, Issue 10, pp. 1162-1164 (2005)
http://dx.doi.org/10.1364/OL.30.001162


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Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

© 2005 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(110.4500) Imaging systems : Optical coherence tomography
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(180.3170) Microscopy : Interference microscopy

Citation
Michael A. Choma, Audrey K. Ellerbee, Changhuei Yang, Tony L. Creazzo, and Joseph A. Izatt, "Spectral-domain phase microscopy," Opt. Lett. 30, 1162-1164 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-10-1162

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