Spectral-domain phase microscopy
Optics Letters, Vol. 30, Issue 10, pp. 1162-1164 (2005)
http://dx.doi.org/10.1364/OL.30.001162
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Abstract
Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.
© 2005 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(110.4500) Imaging systems : Optical coherence tomography
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(180.3170) Microscopy : Interference microscopy
Citation
Michael A. Choma, Audrey K. Ellerbee, Changhuei Yang, Tony L. Creazzo, and Joseph A. Izatt, "Spectral-domain phase microscopy," Opt. Lett. 30, 1162-1164 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-10-1162
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