Abstract

Surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques are integrated in a biosensing imaging system for measuring the two-dimensional spatial phase variation caused by biomolecular interactions on a sensing chip without the need for additional labeling. The common-path PSI technique has the advantage of long-term stability, even when it is subjected to external disturbances. Hence the system meets the requirements of the real-time kinetic studies involved in biomolecular interaction analysis. The proposed SPR–PSI imaging system demonstrates a detection limit of a $2\times {10}^{-7}$ refractive-index change, a long-term phase stability of $2.5\times {10}^{-4}\pi \mathrm{rms}$ for $4\mathrm{h}$, and a spatial phase resolution of ${10}^{-3}\pi$ with a lateral resolution of $100\mu \mathrm{m}$.

© 2005 Optical Society of America

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