OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 30, Iss. 13 — Jul. 1, 2005
  • pp: 1686–1688

Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing

Eran Tal, Dan Oron, and Yaron Silberberg  »View Author Affiliations

Optics Letters, Vol. 30, Issue 13, pp. 1686-1688 (2005)

View Full Text Article

Acrobat PDF (249 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



By introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain video-rate images with depth resolution similar to point-by-point scanning multiphoton microscopy while mechanically scanning in only one dimension. This is achieved by temporal focusing of the illumination pulse: The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration (and highest peak intensity) at the focal plane before stretching again beyond it. This method is applied to produce, in a simple and scalable setup, video-rate two-photon excitation fluorescence images of Drosophila egg chambers with nearly 100,000 effective pixels and 1.5 µm depth resolution.

© 2005 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(320.5540) Ultrafast optics : Pulse shaping

Eran Tal, Dan Oron, and Yaron Silberberg, "Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing," Opt. Lett. 30, 1686-1688 (2005)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  2. G. J. Brakenhoff, J. Squier, T. Norris, A. C. Bliton, M. H. Wade, and B. Athey, J. Microsc. (Oxford) 181, 253 (1995).
  3. D. Oron and Y. Silberberg, J. Opt. Soc. Am. B 21, 1964 (2004).
  4. A. H. Buist, M. Muller, J. Squier, and G. J. Brakenhoff, J. Microsc. (Oxford) 192, 217 (1998).
  5. J. Bewersdorf, R. Pick, and S. W. Hell, Opt. Lett. 23, 655 (1998).
  6. T. Nielsen, M. Fricke, D. Hellweg, and P. Andersen, J. Microsc. (Oxford) 201, 368 (2001).
  7. D. Oron, E. Tal, and Y. Silberberg, Opt. Express 13, 1468 (2005).
  8. O. E. Martinez, IEEE J. Quantum Electron. 23, 59 (1987).
  9. A. Hopt and E. Neher, Biophys. J. 80, 2029 (2001).
  10. E. Stelzer, S. Hell, S. Lindek, R. Stricker, R. Pick, C. Storz, G. Ritter, and N. Salmon, Opt. Commun. 104, 223 (1994).
  11. G. Peleg, A. Lewis, O. Bouevitch, L. Loew, D. Parnas, and M. Linial, Bioimaging 4, 215 (1996).
  12. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997).
  13. M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, J. Microsc. (Oxford) 191, 266 (1998).
  14. M. D. Duncan, J. Reintjes, and T. J. Manuccia, Opt. Lett. 7, 350 (1982).
  15. A. Zumbusch, G. R. Holtom, and X. S. Xie, Phys. Rev. Lett. 82, 4142 (1999).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited