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Optics Letters

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  • Vol. 30, Iss. 17 — Sep. 1, 2005
  • pp: 2266–2268

Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform

Markus Burkhardt, Katrin G. Heinze, and Petra Schwille  »View Author Affiliations


Optics Letters, Vol. 30, Issue 17, pp. 2266-2268 (2005)
http://dx.doi.org/10.1364/OL.30.002266


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Abstract

We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complex biomolecular dynamics on a single molecule level.

© 2005 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

Citation
Markus Burkhardt, Katrin G. Heinze, and Petra Schwille, "Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform," Opt. Lett. 30, 2266-2268 (2005)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-30-17-2266


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