We present a Mie theory-based analysis of angularly resolved light scattering from intact cells that involves the product of a particle size distribution rho and the scattering cross section sigma. The analysis shows that the ability to size intracellular organelles depends strongly upon the assumed functional form of the particle size distribution; however, the product sigma rho extracts the dominant light scatterers regardless of this choice. We examine goniometer measurements from our own and another laboratory and find that this analysis reconciles seemingly conflicting results and confirms that mitochondrial-sized organelles dominate scattering from intact cells in the angular range 5°-90°.
© 2005 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
Jeremy D. Wilson and Thomas H. Foster, "Mie theory interpretations of light scattering from intact cells," Opt. Lett. 30, 2442-2444 (2005)