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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 13 — Jul. 1, 2006
  • pp: 2015–2017

Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells

Kun Chen, Yejun Qin, Feng Zheng, Menghong Sun, and Daren Shi  »View Author Affiliations


Optics Letters, Vol. 31, Issue 13, pp. 2015-2017 (2006)
http://dx.doi.org/10.1364/OL.31.002015


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Abstract

A single-cell diagnostic technique for epithelial cancers is developed by utilizing laser trapping and Raman spectroscopy to differentiate cancerous and normal epithelial cells. Single-cell suspensions were prepared from surgically removed human colorectal tissues following standard primary culture protocols and examined in a near-infrared laser-trapping Raman spectroscopy system, where living epithelial cells were investigated one by one. A diagnostic model was built on the spectral data obtained from 8 patients and validated by the data from 2 new patients. Our technique has potential applications from epithelial cancer diagnosis to the study of cell dynamics of carcinogenesis.

© 2006 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.4730) Medical optics and biotechnology : Optical pathology
(170.5660) Medical optics and biotechnology : Raman spectroscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 12, 2006
Manuscript Accepted: April 13, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Kun Chen, Yejun Qin, Feng Zheng, Menghong Sun, and Daren Shi, "Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells," Opt. Lett. 31, 2015-2017 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-13-2015


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