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Optics Letters

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  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 14 — Jul. 15, 2006
  • pp: 2157–2159

Multidimensional single-molecule imaging in live cells using total-internal-reflection fluorescence microscopy

S. E.D. Webb, S. R. Needham, S. K. Roberts, and M. L. Martin-Fernandez  »View Author Affiliations


Optics Letters, Vol. 31, Issue 14, pp. 2157-2159 (2006)
http://dx.doi.org/10.1364/OL.31.002157


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Abstract

We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation.

© 2006 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 24, 2006
Revised Manuscript: April 25, 2006
Manuscript Accepted: April 27, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Citation
S. E. D. Webb, S. R. Needham, S. K. Roberts, and M. L. Martin-Fernandez, "Multidimensional single-molecule imaging in live cells using total-internal-reflection fluorescence microscopy," Opt. Lett. 31, 2157-2159 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-14-2157


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