Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.
© 2006 Optical Society of America
Medical Optics and Biotechnology
Original Manuscript: May 30, 2006
Manuscript Accepted: July 3, 2006
Published: August 25, 2006
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics
Niyom Lue, Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld, "Live cell refractometry using microfluidic devices," Opt. Lett. 31, 2759-2761 (2006)