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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 18 — Sep. 15, 2006
  • pp: 2759–2761

Live cell refractometry using microfluidic devices

Niyom Lue, Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld  »View Author Affiliations


Optics Letters, Vol. 31, Issue 18, pp. 2759-2761 (2006)
http://dx.doi.org/10.1364/OL.31.002759


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Abstract

Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.

© 2006 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 30, 2006
Manuscript Accepted: July 3, 2006
Published: August 25, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Niyom Lue, Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld, "Live cell refractometry using microfluidic devices," Opt. Lett. 31, 2759-2761 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-18-2759

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