Dynamics of cell membranes and the underlying cytoskeletons observed by noninterferometric widefield optical profilometry and fluorescence microscopy
Optics Letters, Vol. 31, Issue 19, pp. 2873-2875 (2006)
http://dx.doi.org/10.1364/OL.31.002873
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Abstract
Combining the noninterferometric wide-field optical profilometry technique with fluorescence microscopy, we observe the membrane activities of a living cell as well as the structures of its cytoskeletons. The membrane ripples of a lamellipodium are related to similar structures of the underlying actin filaments. However, we find the ripples appear prior to and disappear later than the corresponding actin filament structures, which supports the elastic Brownian ratchet model of cell motility. In addition, we measure the three-dimensional movement of a fibronectin-coated latex bead on the membrane. The bead motion is determined by the movement and branching of the actin molecules on the filament, as well as by the displacement of the filament itself.
© 2006 Optical Society of America
OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.2520) Microscopy : Fluorescence microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: May 31, 2006
Revised Manuscript: July 24, 2006
Manuscript Accepted: July 26, 2006
Published: September 11, 2006
Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Chun-Chieh Wang, Jiunn-Yuan Lin, Hsiao-Chao Chen, and Chau-Hwang Lee, "Dynamics of cell membranes and the underlying cytoskeletons observed by noninterferometric widefield optical profilometry and fluorescence microscopy," Opt. Lett. 31, 2873-2875 (2006)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-31-19-2873
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