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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 31, Iss. 9 — May. 1, 2006
  • pp: 1274–1276

Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation

F. Chasles, B. Dubertret, and A. C. Boccara  »View Author Affiliations

Optics Letters, Vol. 31, Issue 9, pp. 1274-1276 (2006)

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We present a technique for imaging fluorescent particles based on the axial modulation of the objective’s focal plane position. This technique provides full-field optical sectioning and can be used to localize the fluorophores in three dimensions. We describe the technique and apply it to image 200 nm diameter fluorescent beads immobilized in a gel. We show that full-field optical sectioning is obtained and that the beads are localized with a precision of 10 nm in the transverse plane and 14 nm in the axial direction.

© 2006 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: November 30, 2005
Revised Manuscript: February 1, 2006
Manuscript Accepted: February 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

F. Chasles, B. Dubertret, and A. C. Boccara, "Full-field optical sectioning and three-dimensional localization of fluorescent particles using focal plane modulation," Opt. Lett. 31, 1274-1276 (2006)

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