Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces
Optics Letters, Vol. 32, Issue 10, pp. 1308-1310 (2007)
http://dx.doi.org/10.1364/OL.32.001308
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Abstract
Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein monolayers adsorbed on metal surfaces when surface plasmon resonance effects are utilized. The measured data, expressed in ellipsometric angles ψ and Δ are of several orders of magnitude larger in comparison with those from similar experiments performed with traditional ellipsometry. TIRE in spectral mode opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer microstructure.
© 2007 Optical Society of America
OCIS Codes
(120.2130) Instrumentation, measurement, and metrology : Ellipsometry and polarimetry
(160.4760) Materials : Optical properties
(160.4890) Materials : Organic materials
(240.0310) Optics at surfaces : Thin films
(240.6680) Optics at surfaces : Surface plasmons
(300.6550) Spectroscopy : Spectroscopy, visible
ToC Category:
Optics at Surfaces
History
Original Manuscript: November 10, 2006
Revised Manuscript: January 26, 2007
Manuscript Accepted: February 12, 2007
Published: April 17, 2007
Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics
Citation
Michal Poksinski and Hans Arwin, "Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces," Opt. Lett. 32, 1308-1310 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-10-1308
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