Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation
Optics Letters, Vol. 32, Issue 12, pp. 1680-1682 (2007)
http://dx.doi.org/10.1364/OL.32.001680
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Abstract
We developed a compact polarization-mode converter for microscopy to control three-dimensional polarization at the focus. The converter consisted of two homogeneously aligned liquid-crystal spatial light modulators with eight independently controllable electrodes (segments), and a quarter-waveplate. The converter converted a linearly polarized beam to three polarization modes: two orthogonal linear polarizations and a pseudo-radial polarization. We applied the converter to second-harmonic-generation microscopy and demonstrated the detection of three-dimensional molecular orientation.
© 2007 Optical Society of America
OCIS Codes
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
ToC Category:
Microscopy
History
Original Manuscript: January 12, 2007
Manuscript Accepted: March 9, 2007
Published: June 5, 2007
Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics
Citation
Keisuke Yoshiki, Kanamaru Ryosuke, Mamoru Hashimoto, Nobuyuki Hashimoto, and Tsutomu Araki, "Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation," Opt. Lett. 32, 1680-1682 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-12-1680
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