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Optics Letters

Optics Letters


  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 15 — Aug. 1, 2007
  • pp: 2121–2123

Two-photon standing-wave fluorescence correlation spectroscopy

Kerry M. Hanson, Sara K. Davis, and Christopher J. Bardeen  »View Author Affiliations

Optics Letters, Vol. 32, Issue 15, pp. 2121-2123 (2007)

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A fluorescence correlation spectroscopy experiment that combines two-photon excitation and a standing-wave interference pattern is presented. The experimental correlation function can be analyzed using a simple expression involving (1) an exponential decay with time constant τ f , which reflects diffusion across the interference fringes, and (2) a longer-lived decay with time constant τ ω , which reflects diffusion in and out of the focal spot. The diffusion of Rhodamine 110 in water and ethylene glycol is measured using this method. The ability to simultaneously measure diffusion on two different time and lengthscales makes this experiment especially useful in environments where anomalous diffusion is suspected.

© 2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy
(300.6410) Spectroscopy : Spectroscopy, multiphoton

ToC Category:

Original Manuscript: March 20, 2007
Revised Manuscript: April 26, 2007
Manuscript Accepted: May 30, 2007
Published: July 20, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Kerry M. Hanson, Sara K. Davis, and Christopher J. Bardeen, "Two-photon standing-wave fluorescence correlation spectroscopy," Opt. Lett. 32, 2121-2123 (2007)

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  1. M. P. Sheetz, S. Turney, H. Qian, and E. L. Elson, Nature 340, 284 (1989). [CrossRef] [PubMed]
  2. T. Schmidt, G. J. Schutz, W. Baumgartner, H. J. Gruber, and H. Schindler, Proc. Natl. Acad. Sci. U.S.A. 93, 2926 (1996). [CrossRef] [PubMed]
  3. A. Yildiz, J. N. Forkey, S. A. Mckinney, T. Ha, Y. E. Goldman, and P. R. Selvin, Science 300, 2061 (2003). [CrossRef] [PubMed]
  4. V. Levi, Q. Ruan, M. Plutz, A. S. Belmont, and E. Gratton, Biophys. J. 89, 4275 (2005). [CrossRef] [PubMed]
  5. D. Magde, E. Elson, and W. W. Webb, Phys. Rev. Lett. 29, 705 (1972). [CrossRef]
  6. E. L. Elson and D. Magde, Biopolymers 13, 1 (1974). [CrossRef]
  7. E. L. Elson, D. Magde, and W. W. Webb, Biopolymers 13, 29 (1974). [CrossRef] [PubMed]
  8. S. Maiti, U. Haupts, and W. W. Webb, Proc. Natl. Acad. Sci. U.S.A. 94, 11753 (1997). [CrossRef] [PubMed]
  9. K. M. Berland, P. T. C. So, and E. Gratton, Biophys. J. 68, 694 (1995). [CrossRef] [PubMed]
  10. P. Schwille, U. Haupts, S. Maiti, and W. W. Webb, Biophys. J. 77, 2251 (1999). [CrossRef] [PubMed]
  11. M. Hattori, H. Shimizu, and H. Yokoyama, Rev. Sci. Instrum. 67, 4064 (1996). [CrossRef]
  12. R. L. Hansen, X. R. Zhu, and J. M. Harris, Anal. Chem. 70, 1281 (1998). [CrossRef] [PubMed]
  13. T. Sonehara, K. Kojima, and T. Irie, Anal. Chem. 74, 5121 (2002). [CrossRef] [PubMed]
  14. P.-F. Lenne, E. Etienne, and H. Rigneault, Appl. Phys. Lett. 80, 4106 (2002). [CrossRef]
  15. H. Rigneault and P.-F. Lenne, J. Opt. Soc. Am. B 20, 2203 (2003). [CrossRef]
  16. D. Margineantu, R. A. Capaldi, and A. H. Marcus, Biophys. J. 79, 1833 (2000). [CrossRef] [PubMed]
  17. S. Hell and E. H. K. Stelzer, J. Opt. Soc. Am. B 9, 2159 (1992).
  18. A. Egner, S. Jakobs, and S. W. Hell, Proc. Natl. Acad. Sci. U.S.A. 99, 3370 (2002). [CrossRef] [PubMed]
  19. J. Chen and K. Midorikawa, Opt. Lett. 29, 1354 (2004). [CrossRef] [PubMed]
  20. S. K. Davis and C. J. Bardeen, Rev. Sci. Instrum. 73, 2128 (2002). [CrossRef]
  21. S. K. Davis and C. J. Bardeen, Biophys. J. 86, 555 (2004). [CrossRef]
  22. E. B. Brown, E. S. Wu, W. Zipfel, and W. W. Webb, Biophys. J. 77, 2837 (1999). [CrossRef] [PubMed]
  23. J. D. Müller, Y. Chen, and E. Gratton, Methods Enzymol. 361, 69 (2003). [CrossRef] [PubMed]
  24. W. S. Jones and W. S. Pamplin, in Glycols, G.O.Curme and F.Johnston, eds. (Reinhold, 1953), p. 57.
  25. M. Lim and C. Saloma, Appl. Opt. 42, 3398 (2003). [CrossRef] [PubMed]

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