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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 23 — Dec. 1, 2007
  • pp: 3408–3410

Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

Dylan M. Owen, Egidijus Auksorius, Hugh B. Manning, Clifford B. Talbot, Pieter A. A. de Beule, Christopher Dunsby, Mark A. A. Neil, and Paul M. W. French  »View Author Affiliations


Optics Letters, Vol. 32, Issue 23, pp. 3408-3410 (2007)
http://dx.doi.org/10.1364/OL.32.003408


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Abstract

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.

© 2007 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.2520) Microscopy : Fluorescence microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 28, 2007
Revised Manuscript: October 25, 2007
Manuscript Accepted: October 29, 2007
Published: November 21, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Dylan M. Owen, Egidijus Auksorius, Hugh B. Manning, Clifford B. Talbot, Pieter A. A. de Beule, Christopher Dunsby, Mark A. A. Neil, and Paul M. W. French, "Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source," Opt. Lett. 32, 3408-3410 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-23-3408

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