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Optics Letters

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  • Vol. 32, Iss. 5 — Mar. 1, 2007
  • pp: 503–505

Imaging subcellular scattering contrast by using combined optical coherence and multiphoton microscopy

Shuo Tang, Chung-Ho Sun, Tatiana B. Krasieva, Zhongping Chen, and Bruce J. Tromberg  »View Author Affiliations


Optics Letters, Vol. 32, Issue 5, pp. 503-505 (2007)
http://dx.doi.org/10.1364/OL.32.000503


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Abstract

The structural origin of scattering contrast from single cells is examined by using a combined optical coherence and multiphoton microscope based on a 12 fs Ti:sapphire source and a 0.95 NA objective. High-resolution coherence-gated scattering images from single cells are coregistered and compared with two-photon-excited fluorescence images. Scattering contrast is observed from mitochondria, plasma membrane, actin filaments, and the boundary between cytoplasm and nucleus. There is little contribution to scattering from regions inside the nuclear core. These results confirm that light scattering signals from specific subcellular structures can be visualized by using coherent reflectance geometry.

© 2007 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(180.2520) Microscopy : Fluorescence microscopy
(290.0290) Scattering : Scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 12, 2006
Revised Manuscript: November 22, 2006
Manuscript Accepted: November 27, 2006
Published: February 2, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Shuo Tang, Chung-Ho Sun, Tatiana B. Krasieva, Zhongping Chen, and Bruce J. Tromberg, "Imaging subcellular scattering contrast by using combined optical coherence and multiphoton microscopy," Opt. Lett. 32, 503-505 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-5-503


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