Abstract

In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of $2\mu \mathrm{m}$. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack–Hartmann wavefront sensor that detects fluorescent wavefront.

© 2007 Optical Society of America

Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice

Daniel J. Wahl, Yifan Jian, Stefano Bonora, Robert J. Zawadzki, and Marinko V. Sarunic
Biomed. Opt. Express 7(1) 1-12 (2016)

Adaptive optics scanning laser ophthalmoscopy and optical coherence tomography (AO-SLO-OCT) system for in vivo mouse retina imaging

Pengfei Zhang, Daniel J. Wahl, Jacopo Mocci, Eric B. Miller, Stefano Bonora, Marinko V. Sarunic, and Robert J. Zawadzki
Biomed. Opt. Express 14(1) 299-314 (2023)

Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina

Robert J. Zawadzki, Pengfei Zhang, Azhar Zam, Eric B. Miller, Mayank Goswami, Xinlei Wang, Ravi S. Jonnal, Sang-Hyuck Lee, Dae Yu Kim, John G. Flannery, John S. Werner, Marie E. Burns, and Edward N. Pugh
Biomed. Opt. Express 6(6) 2191-2210 (2015)

Adaptive optics retinal imaging in the living mouse eye

Ying Geng, Alfredo Dubra, Lu Yin, William H. Merigan, Robin Sharma, Richard T. Libby, and David R. Williams
Biomed. Opt. Express 3(4) 715-734 (2012)

In vivo two-photon imaging of the mouse retina

Robin Sharma, Lu Yin, Ying Geng, William H. Merigan, Grazyna Palczewska, Krzysztof Palczewski, David R. Williams, and Jennifer J. Hunter
Biomed. Opt. Express 4(8) 1285-1293 (2013)