Abstract

A time-resolved spectroscopic imaging system is built to study the fluorescence characteristics of nicotinamide adenine dinucleotide (NADH), an important metabolic coenzyme and endogenous fluorophore in cells. The system provides a unique approach to measure fluorescence signals in different cellular organelles and cytoplasm. The ratios of free over protein-bound NADH signals in cytosol and nucleus are slightly higher than those in mitochondria. The mitochondrial fluorescence contributes about 70% of overall cellular fluorescence and is not a completely dominant signal. Furthermore, NADH signals in mitochondria, cytosol, and the nucleus respond to the changes of cellular activity differently, suggesting that cytosolic and nuclear fluorescence may complicate the well-known relationship between mitochondrial fluorescence and cellular metabolism.

© 2008 Optical Society of America

Two-photon autofluorescence microscopy of multicolor excitation

Dong Li, Wei Zheng, and Jianan Y. Qu
Opt. Lett. 34(2) 202-204 (2009)

Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding

Thomas H. Chia, Anne Williamson, Dennis D. Spencer, and Michael J. Levene
Opt. Express 16(6) 4237-4249 (2008)

Sensing cell metabolism by time-resolved autofluorescence

Yicong Wu, Wei Zheng, and Jianan Y. Qu
Opt. Lett. 31(21) 3122-3124 (2006)

Nicotinamide effects on the metabolism of human fibroblasts and keratinocytes assessed by quantitative, label-free fluorescence imaging

Zhiyi Liu, Chung-Yi Chiang, John Nip, Lin Feng, Yang Zhang, Sheila Rocha, and Irene Georgakoudi
Biomed. Opt. Express 12(10) 6375-6390 (2021)

In vivo monitoring of protein-bound and free NADH during ischemia by nonlinear spectral imaging microscopy

Jonathan A. Palero, Arjen N. Bader, Henriëtte S. de Bruijn, Angélique van der Ploeg van den Heuvel, Henricus J. C. M. Sterenborg, and Hans C. Gerritsen
Biomed. Opt. Express 2(5) 1030-1039 (2011)