Holographic gratings formed in thick phenanthrenquinone- (PQ-) doped poly(methyl methacrylate) (PMMA) can be made to have narrowband spectral and spatial transmittance filtering properties. We present the design and performance of angle-multiplexed holographic filters formed in PQ-PMMA at $488\mathrm{nm}$ and reconstructed with a LED operated at $\sim 630\mathrm{nm}$ . The dark delay time between exposure and the preillumination exposure of the polymer prior to exposure of the holographic area are varied to optimize the diffraction efficiency of multiplexed holographic filters. The resultant holographic filters can enhance the performance of four-dimensional spatial–spectral imaging systems. The optimized filters are used to simultaneously sample spatial and spectral information at five different depths separated by $50\mathrm{\mu }\mathrm{m}$ within biological tissue samples.